Alien Instinct is PlayHouseFun's movie-spoof of "Killer Instinct".
- Fulgore - Nasty Jack (The New Adventures of Winnie the Pooh)
- Spinal - Mortimer Mouse (Mickey Mouse Works)
- Glacius - Oscar the Grouch (Sesame Street)
- Cinder/Ben Ferris - Grand Duke of Owls (Rock-a-Doodle)
- Riptor - Eeyore (Winnie the Pooh)
- Sabrewulf - Bruce (Finding Nemo)
- Jago - Orbulon (WarioWare)
- B. Orchid - Terra (WarioWare)
- TJ Combo - Dribble (WarioWare)
- Chief Thunder - Itchy (All Dogs Go to Heaven)
- Eyedol - Petey Piranha (Mario Bros)
- Director - Wilbur (Charlotte's Web)
- Tiger Spirit - Boog (Open Season)
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