Ami is the animated counterpart of Ami Onuki. She is one of the members with Yumi Yoshimura and one of the two main protagonists of the series. She is voiced by Andrea Libman.
Ami has apricot skin, hot pink eyes and long hot pink hair that goes down to her waist. She also partially ties her hair into buns. She also usually wears a flower hairclip. She has black mascara. She favors the pop music of the '60s and wears a light pink go-go dress with yellow flowers, a silver-and-emerald bracelet on her right wrist, an orange beaded bracelet on her left wrist, and white knee-high go-go boots with the zipper lying where her feet is located.
Ami is known for her girly-girl and goody-two-shoes personality. As the optimist, Ami always looks on the bright side of things and finds a solution to every situation she is involved in. She loves rainbows, bunnies (cute animals), sparkles and anything else typically pretty, which is the opposite of Yumi, who likes things more targeted to a tomboyish and unisex demographic.
She also cares a lot about how other people think, and tries not to be selfish. This makes Ami willing to help everyone and find solutions for anyone he cares. On the other side, however, she also hides her real mind and opinions in order to never disappoint others. She warmly welcomes new faces such as Pinkie Pie and Jody Mitchell. True to peppy and outgoing personality she represents, she loves to make everybody smile and laugh.
Ami can play her drums with her bare feet, as seen in both "Ami Goes Bad" and "Mean Machine." Some episodes such as "Julie AmiYumi" shows that she is also capable of playing electric guitar like Yumi, who is main guitarlist of the band. Ami can crush a handheld gaming device with her hand as seen in "The Legend of Mei Pie". She is also a ninja like Yumi, though not as skillful as Yumi. Ami can also throw things far away as seen in "Gridiron Maidens", because of always pounding on the drums. She also swings her microphone to make it fly like a helicopter to get over big gaps as seen in the game "Hi Hi Puffy AmiYumi: Kaznapped!".
- Her real-life counterpart does not have hot pink hair, instead she has dyed strawberry-blonde hair and does not wear pigtailed buns and a daisy hairclip.
- Rodney Greenblatt’s (the character designer for the PlayStation game PaRappa the Rapper) design for Ami Onuki looks unlike her Cartoon Network counterpart. The design looks exactly like her real-life counterpart.
- Her design is somewhat similar to Agent Xero to the failed pilot The Modifyers.
- Her voice actress Andrea Libman also voiced Pinkie Pie from the Hasbro show My Little Pony: Friendship is Magic.
- Her design is very similar to the show version of Ami in "Hi Hi Puffy AmiYumi" and the other version of her.
- Her personality is just the same as Lyloo Lasti, Starfire from Teen Titans and Teen Titans Go!, Pinkie Pie from My Little Pony, Bubbles from The Powerpuff Girls, and Cartoon Network's 2004 counterpart.