Red Team/Good Team:
Heavy Weapons Guy - Homer Simpson (The Simpsons)
Scout - SpongeBob SquarePants
Soldier - Scrooge McDuck (DuckTales)
Spy - Villager (Minecraft)
Engineer - Brian Griffin (Family Guy)
Medic - Nurse Joy (Pokémon)
Demoman - Donkey (Shrek)
Sniper - Bender (Futurama)
Pyro - Kenny (South Park)
Blu Team/Bad Team:
Heavy Weapons Guy - Fat Tony (The Simpsons)
Scout - DoodleBob (SpongeBob SquarePants)
Soldier - Flintheart Glomgold (DuckTales)
Spy - Vindicator (Minecraft)
Engineer - New Brian (Family Guy)
Medic - Jessie (Pokémon)
Demoman - Lord Farquaad (Shrek)
Sniper - Roberto (Futurama)
Pyro - Princess Kenny (South Park: The Stick of Truth)
Other Characters:
Administrator "Announcer" - Stella (Minecraft: Story Mode)
AP-SAP - Spike (MLP:FIM)
Monoculus - Dragon (Shrek)
Bombinomicon - Bendy (Bendy and the Ink Machine)
Merasmus - Discord (MLP:FIM)
Redmond Mann - Jack Skellington (The Nightmare Before Christmas)
Blutarch Mann - Oogie Boogie (The Nightmare Before Christmas)
Pumpkin Bomb - Electrode (Pokémon)
Nieodemus Mann - Grim (The Grim Adventures of Billy and Mandy)
Ms. Pauling - Sandy Cheeks (SpongeBob SquarePants)
Archimedes the Undying - Wobbuffet (Pokémon)