The Great Equestria Girl Detective is Twilight'sSpaceStar17's second movie-spoof of "The Great Mouse Detective":
Cast:[]
- Human Twilight Sparkle (Me "My Little Pony: Equestria Girls") as Basil of Baker Street
- Human Rainbow Dash, Human Fluttershy, Human Applejack, Human Rarity, Human Pinkie Pie and Spike the Dog (My Little Pony: Equestria Girls) as Extras with Human Twilight Sparkle
- Gru (Despicable Me) as Dr. Dawson
- Mavis (Hotel Transylvania) as Olivia Flaversham
- Count Dracula (Hotel Transylvania) as Hiram Flaversham
- Jake Long the Dragon (American Dragon: Jake Long) as Toby the Dog
- Principal Celestia and Vice Principal Luna (as an extra) (My Little Pony: Equestria Girls) as Mrs. Judson
- Sunset Shimmer (My Little Pony: Equestria Girls) as Professor Ratigan
- Human Snips and Human Snails (as an extra) (My Little Pony: Equestria Girls) as Fidget the Bat
- Venom and His Villain Buddies as Ratigan's Henchmen
- Sabor (Tarzan) as Felicia
- ZigZag (The Thief and The Cobbler) as Bartholomew
- Peter Griffin (Family Guy) as Juggling Octopus
- Abbey Bominable (Monster High) as Barmaid
- Megamind and Minion (Megamind) as Frog and Salamander on Bike
- Kimiko (Xiaolin Showdown/Xiaolin Chronicles) as Miss Kitty
- Tomoko (Xiaolin Chronicles), Raven Queen (Ever After High), Anna and Elsa (Frozen) as Miss Kitty's Sisters
- Eden (Aladdin (TV Series)) as Queen Moustoria