Luigi is Mario's slightly younger, taller twin brother and the deuteragonist in the Super Mario franchise. He is timid and a bit cowardly, but he overcomes his fears and wills to protect his homeland with his brother's help.
- Luigi played as Fiddler Pig in The Three Pesky Plumbers and The Big Bad Koopa (The Big Bad Wolf 1934).
- Luigi played as Luigi in Animateds (Cars).
- Luigi played Pumbaa in The Lombax King, The Cartoon King and The Dog King (StrongTed201 Style).
- Luigi played as himself in The Italian King.
- Luigi played as Fetcher in Fox Run.
- Luigi played Spongebob in LUIGIBOB LPANTS.
- Luigi played Queen Trash's Stretchmen in The Adventures of Bambi in Grouchland.
- Luigi played Himself in Yu-Gi-Oh! 5D's (Yusei64 Style).
- Luigi played Egon Spengler in Creaturebusters (Ghostbusters), The Real Creaturebusters (The Real Ghostbusters), Creaturebusters II (Ghostbusters II) and Extreme Creaturebusters (Extreme Ghostbusters).
- Luigi played Aladdin in Luigiladdin (Videogame Style).
- Luigi played Boog Shlizetti in ErnieBoy and Bert Bert.
- Luigi played Ziggy in Lazytown.
- Luigi played Philoctetes in Mariocules.
- Luigi Played Kowalski in Madagascar (Gabriel Adam Pictures Style).
- Luigi Played Mr. Pricklepants in Animation Story 3 (2010), Animation Story 4 (2019) and Orbulon Asks a Question.
- Luigi played Genie in Marioladdin (CartoonFanBoy91).
- Luigi played Otto in Link Hood.
- Luigi played Chubby in The Pebble And The Plumber (PeachyNova Productions Style).
- Luigi Plays Hawkeye In Captain Mario:Civil War.
- Luigi Plays Ferb In Mario & Luigi:Mission Marvel.
- Luigi Plays Flying Scotsman In Eric Cartman And Friends.
- Luigi Plays Buzz Lightyear in Toy Story (Mario Fan 92 Style).
- Luigi Plays Tow Mater in Cars (Mario Fan 92 Style).
- Luigi Plays Donkey in Mario (Shrek) (Mario Fan 92 Style).
- Luigi plays Elmo in Luigi's Mansion: Kids' Favorite Songs 2.
- Luigi plays Joshamee Gibbs in Toons of the Caribbean.
- Luigi plays Ragetti in Ash Ketchum of the Caribbean and Roy of the Caribbean.
- In Super Bob Bros., he is played by Larry the Cucumber
- in Planet Luigi he plays Sheen
- In Super Charlie BrownRockz Bros. Series He plays Ted Wiggins
- In Mario & Luigi:Mission Marvel He Plays Ferb
- In Super Gordon, Super Smash Bros Ultimate (Crossover Edition), And Racist Gordon He Plays Flying Scotsman
- Like Mario, he was born in Scandicci, Italy.
- His appearances in the Stephen Squirrelsky and Friends' Film Spoof Travels are The Secret of NIMH (Nikkdisneylover8390's Human Style), Nikki's Adventures of Sing Along Songs Episode 6, The Little Mer-Lioness II: Return to the Sea, Skunkules, Home on the Range (DalMatian Tunes' Style), Kermit's Winter Wonderland, The Little Jungle Boy, Kermiladdin 2: The Return of McLeach, AiAi the Monkey and A Day for George, The Reindeer and the Deer, Animal Story 2 (Stephen Druschke's Style), AiAi's Grand Adventure: The Search for Tails, A Cartoon Character's Life, Nikki's Adventures of Sing Along Songs Episode 7, Here Comes Winnie the Pooh, Animals, Inc. (Dragon Rockz's Style), Simba 2, Miss Bianca and the Bernard 2: Fievel's Adventure, FernGully: The Last Rainforest (Dragon Rockz's Style), A Pooh in Central Park, Bubblesrella 2: Dreams Come True, The Brave Little Piglet, Kung Fu Owen, Nikki's Adventures of Sing Along Songs Episode 8, The Thomas O'Malley Movie, Kermit Returns, Fievel the Red Nosed Mouse and The Island of Misfits, Nikki's Adventures of Sing Along Songs Episode 9, A Cartoon Character's Life Activity Center, Cloudy with a Chance of Meatballs (Justin Quintanilla's Style), Finding Alvin (J.B. Eagle's Style), Animated Tale (TheTure DisneyKing's Style), The Forest Book 2 (TrainBoy43's Style), Nikki's Adventures of Sing Along Songs Episode 10, Madagascar (FamousImagination50's Style), Sleeping Beauty (Justin Quintanilla's Style), Aurorastasia, Despicable Me (Justin Quintanilla's Style), We're Back!: A Jungle Animal's Story, Jimmynocchio, and The Wizard of Oz (Uranimated18's Style) and will even be in more movie spoof travels.