Batman/Bruce Wayne as Flik
Barbara Gordon/Batgirl as Princess Atta
Commissioner James Gordon as The Queen
Alfred Pennyworth as Cornelius
The Joker as Hopper
Harley Quinn as Dot
Superman as Francis
Robin/Dick Grayson as Heimlich
Poison Ivy as Rosie
Green Lantern as Slim
Clayface as Dim
Riddler and Scarecrow as Tuck and Roll
Flash as Manny
Catwomen as Gyspy
The Penguin as P.T Flea
Killer Croc as Molt
Dracula and Kraken as The Flies
Phantom Zone Criminals as Hopper's Grasshoppers
Lord Voldemort as Thumper
Sauron and King Kong as Axel and Loco