Cast:
- Kuzco - Maya Hathaway
- Kuzco (Llama) - Snowball (The Secret Life of Pets)
- Baby Kuzco - Baby Herman (Roger Rabbit)
- Pacha - Santiago Manuel Rodriguez
- Yzma - Harley Quinn (Birds of Prey (2019))
- Kronk - Kyle Scheyd
- Chicha - Marley
- Chaca - Coraline
- Tipo - DJ (Monster House)
- Misty the Llama - Bethany Rabbit
- Bucky the Squirrel - Sandy Cheeks (SpongeBob SquarePants)
- Theme Song Guy - Ms. Amy
- 1st Guard - Lord Rataxes (Babar: The Movie)
- Old Man (aka Rudy) - Forky (Toy Story 4)
- Man for Bride Choosing - Danny (Cats Don't Dance)
- Brides - ???
- Peasant Near Yzma - Dodo (Alice In Wonderland (2010))
- Announcer for Pacha - Grinch (The Grinch)
- Jaguars as themselves
- Crocodiles - Nero and Brutus (The Rescuers)
- Bees - Bees (Roger Rabbit)
- Bird Bingo - Pingu
- Waitress - Nai Nai (Abominable)
- Man giving thumbs up - Rex (Toy Story)
- Chef - Chef Skinner (Ratatouille)
- Birthday Singers - Monkeys Playing Glockenspiel (Baby Einstein: World Animals)
- Llamas - Various Bunnies
- 2 Men at the Checkboard (aka Topo and Ipi) - Shane and David (The Upside Down Show)
- Woman with Pinata - Queen Elinor (Brave)
- Children with Pinata - Hamish, Hubert and Harris (Brave)
- Guards - Rhino Guards (Robin Hood)
- Guard in Warthog Form - Bebop (Teenage Mutant Ninja Turtles 2: Out of the Shadows)
- Guard in Lizard Form - Bruni (Frozen 2)
- Guard in Ostrich Form - Ballerina Ostrich (Fantasia)
- Guard in Octopus Form - Octopus (Baby Einstein)
- Guard in Gorilla Form - King Louie (LA) (The Lion King (2016))
- Guard in Cow Form - Hippo (Baby Einstein)
- Kuzco (Turtle) - Neptune (Baby Einstein)
- Kuzco (Bird) - Tweety Bird (Looney Tunes)
- Kuzco (Whale) - Pinkie Pie (My Little Pony: Friendship is Magic)
- Yzma (Kitten) - Periwinkle (Blue's Clues)
- 3rd Guard - Vachir (Kung Fu Panda)
- Bouncing Owner - E.B.’s Dad (Hop)
- Chicha's Baby - Baby Santiago (aka Santi)
Scenes:
- The Missypants' New Groove (Santiago Style) part 1 - Opening/"Perfect World"
- The Missypants' New Groove (Santiago Style) part 2 - Santiago's Arrival/The Missypants' Advisor
- The Missypants' New Groove (Santiago Style) part 3 - Mayatopia
- The Missypants' New Groove (Santiago Style) part 4 - Harley Quinn's Revenge
- The Missypants' New Groove (Santiago Style) part - A Diabolical Dinner
- The Missypants' New Groove (Santiago Style) part 6 - Finishing the Job
- The Missypants' New Groove (Santiago Style) part 7 - Santiago Returns Home
- The Missypants' New Groove (Santiago Style) part 8 - Demon Rabbit!
- The Missypants' New Groove (Santiago Style) part 9 - Into The Jungle/Santiago To The Rescue
- The Missypants' New Groove (Santiago Style) part 10 - The Transition of Power
- The Missypants' New Groove (Santiago Style) part 11 - Battle at the Bridge
- The Missypants' New Groove (Santiago Style) part 12 - In Hot Pursuit
- The Missypants' New Groove (Santiago Style) part 13 - At Mudka's Meat Hut
- The Missypants' New Groove (Santiago Style) part 14 - A Rabbit Alone/Friends, Finally
- The Missypants' New Groove (Santiago Style) part 15 - Playtime at Santiago's House
- The Missypants' New Groove (Santiago Style) part 16 - The Chase
- The Missypants' New Groove (Santiago Style) part 17 - Harley Quinn Confronts Snowball (aka Maya)
- The Missypants' New Groove (Santiago Style) part 18 - A Mix-Up of Vials
- The Missypants' New Groove (Santiago Style) part 19 - Harley Quinn's Cat Form
- The Missypants' New Groove (Santiago Style) part 20 - A Whole New Groove
- The Missypants' New Groove (Santiago Style) part 21 - End Credits