Cast[]
- Thomas as Scooby Doo - (Thomas and Scooby Doo are both funny, cool, talking animation characters and the main stars)
- Edward as Shaggy Rogers - (Thomas and Edward are good friends, just like Scooby Doo and Shaggy Rogers are)
- James as Fred Jones - (James and Fred Jones are both vain)
- Emily as Daphne Blake - (Emily and Daphne Blake are both beautiful)
- Tillie (from The Little Engine That Could/Casey Jr. and Friends) as Velma Dinkley - (Tillie and Velma Dinkley are both cute)
- Percy as Scrappy Doo - (Thomas and Percy are best friends, just like Scooby Doo and Scrappy Doo are)
- Casey Jr. (from Disney's Dumbo/Casey Jr. and Friends) as Scooby Dum - (Casey Jr. and Scooby Dum are both smart and wise)
- Troublesome Trucks and Z-Stacks (from TUGS) as Villains, Cats, Zombies, Mummies, and The Aliens
- Gordon as Police Officer
- Donald and Douglas as Batman and Robin - (Donald and Douglas are twins, just like Batman and Robin are)
- Oliver as Uncle Nathaniel - (Oliver and Uncle Nathaniel are both western)
- Jebediah (from The Little Engine That Could/Casey Jr. and Friends) as Mr. McKnight
- Johnny (from The Brave Engineer/Casey Jr. and Friends) as Jerry Reed - (Johnny and Jerry Reed are both named begins with the letter 'J')
- Duck as Omar Karam - (Duck and Omar Karam are both western)
- Toots (from Porky's Railroad/Casey Jr. and Friends) as Gibby Norton
- Mavis as Lena Dupree
- Daisy as Simone Lenoir
- Flora as Mrs. Cutler
- Diesel as Ben Ravencroft
- Alfred (from Sodor Dark Times) as Phantom Virus
- Henry as The Mayor
- Annie, Clarabel, and Henrietta as The Hex Girls
- Lorry 1 as Max
- Lorry 2 as Steve
- Lorry 3 as Laura
- Molly as Crystal
- Rosie as Amber
- BoCo as Officer Wembley
- Toby as Professor Kaufman - (Toby and Professor Kaufam are both old, wise, and kind)
- Arthur as Eric
- Smudger as Bill
- Bill and Ben as Laurel and Hardy - (Bill and Ben are twins, just like Laurel and Hardy are)
- The Narrow Gauge Engines as the Harlem Globetrotters
- Toad as Dribbles
- Marge (made up narrow gauge steam engine) as Granny (of the Globetrotters)
- Arry and Bert as Joker and Penguin - (Arry and Bert are twins, just like Joker and Penguin are)
- Neville as Detective Neville - (Neville and Detective Neville are both share the same names)
- Spencer as the Boss
- Hector as Mr. Beamon
- Diesel 10 as the Fisherman
- S.C.Ruffey as the Pig
- Cerberus (from The Little Engine That Could '2011 Film') as Jock
- Iron Duke (from RWS) as Lester
- Belle as Dottie
- Splatter and Dodge as Fake military police officers
- The Chinese Dragon as Witch's Ghost
- The Witch Engine (from Cactus190706) as Sarah Ravencroft
- Dennis as Night Guard
- Jack as Jack - (Jack and Jack are both share the same names)