They were later seen in Crash Twinsanity at the start of the game. They proceeded to kidnap Cortex under the orders of Papu Papu. However, Crash eventually saved him from their clutches.
Their appearance stayed relatively the same in all of their appearances in the series. By Twinsanity, they seem to be more advanced, as they are seen with binoculars and boats. This tribe practises human sacrifice, which is shown when they start chanting when Cortex falls down into the river and falls off the waterfall. If a Tribesman with binoculars see Crash they will start jumping up and down, alerting the other tribesmen and Crash will be hit with spears.
In the "N. Sane Trilogy", remaster of the first the tribesmen repeat the same role which they had in the original. They also appear in Nitro Fueled featured in a cutscene before Papu Papu's challenge, and additionally they can be found in Skull Rock battle arena.