Two years later, Peter Parker's Spider-Man suit had changed differently, the spiders on the front and back were different in design, the red portions of the suit were lighter and the blue portions of the suit were darker. The web pattern was still silver in colour, but brighter.
Spider-Man no more
As Peter was struggling to balance his double life as a college student and a superhero, Peter began to lose his spider powers. Due to an emotional breakdown, he decided to give up being Spider-Man. He tosses his Spider-Man suit in a trash bin and walks away. Days later, a garbage man discovers the discarded suit and brings it to the Daily Bugle where J. Jonah Jameson kept it on a wall (he also temporarily wore the suit, in a deleted scene in Spider-Man 2.1).
Due to a shock of emotion seeing that Doctor Octopus took Mary Jane hostage (in an attempt to lure Spider-Man into a trap), Peter's powers fully return and he dons his suit again by stealing it from the Bugle and leaving a note via a web on the wall where his suit was previously hanged. Through his battle with Doctor Octopus on a train and in his lair, the suit was once again damaged but after the death of Doctor Octopus and the successful rescue of Mary Jane, Peter discarded his damaged suit and wore one of his spare suits.
Six months later, the Spider-Man suit looks the same as the one in the previous film as Peter continues using it to fight crime as the superhero Spider-Man while the people of New York now likes Spider-Man, especially the police and fire department.
While on a date with his now girlfriend Mary Jane Watson, a meteor crashes down, with a mysterious sentient goo, crawling out of it. It attaches itself to Peter's license plate on his motorbike, and later bonds with him while he was in bed.
The symbiote changes the suit from it's friendly red and blue design, to full black/dark grey. The spider on his chest has changed from it's normal look to a more aggresive version. The top four legs now curve inward more, while the bottom legs become longer and are straighter. The back spider also has changed and seemed to have the same design of the front spider. This new suit had enhanced his powers, but it also slowly started to make him arrogant and aggressive. When Peter finds out after the symbiote made him lash out and hit Mary Jane, he got rid of it by using the high frequencies of a bell chime in a church to weaken it, and tear it off of his body.
(An early version of this symbiote suit was entirely black, with the lenses of the eyes being slightly larger, with sharper edges. There were also two possible symbols that they debated using. One appears to be a larger and white version of the symbol they did end up using, and the other looking more like a comic-accurate Venom chest spider. No back spider can be seen, but it's safe to say they would've ended up using the same spider symbol front and back, whichever one they decided to use. The idea and the suit were eventually scrapped because, "The producers didn't want to use it because they felt the latex made Spidey look like he was in a porno").
Return to the Red and Blue suit
After rejecting the black symbiotic suit, Peter went back to his original Spider-Man suit and went off on an attempt to save Mary Jane who was being held hostage by Eddie Brock (who had at this point bonded to the symbiote to become Venom) and Sandman. After a long brutal battle following the deaths of the Venom symbiote, Brock, and Peter's best friend Harry Osborn, the Spider-Man suit was once again damaged but was eventually replaced by another spare suit.