Cast
- Scooby-Doo as Captain America/Steve Rogers
- Shaggy as Iron Man/Tony Stark
- Elliot Blender as Crossbones/Brock Rumlow
- Daphne as Black Widow/Natasha Romanoff
- Fred as Hawkeye/Clint Barton
- Sam (from Roller Ghoster Ride) as Falcon/Sam Wilson
- The Ancient One (from Big Appetite in Little Tokyo) as Winter Soldier/Bucky Barnes
- Lloyd Mbuku/Robbie Kumbazi as Black Panther/T'Challa
- 30 Foot Shaggy as Baron Helmut Zemo
- Chrissie (Secret Six's Mother) as Agent 13/Sharon Carter
- Crawdad Mike's Bus Driver as Thunderbolt Ross
- Burr Batson as Ant-Man/Scott Lang
- Murph (from It's Mean, It's Green, It's the Mystery Machine) as War Machine/James Rhodes
- Crawdad Mike as Vision
- Velma as Scarlet Witch/Wanda Maximoff
- Little Pete the Penguin as Spider-Man/Peter Parker
- Mandy Dinwiddie as Christine Everhart
- Lindsay Pagano as Peggy Carter